|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human PTPRJ ORF mammalian expression plasmid, C-GFPSpark tag||HG13165-ACG|
|Human PTPRJ ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13165-ACR|
|Human PTPRJ ORF mammalian expression plasmid, C-Flag tag||HG13165-CF|
|Human PTPRJ ORF mammalian expression plasmid, C-His tag||HG13165-CH|
|Human PTPRJ ORF mammalian expression plasmid, C-Myc tag||HG13165-CM|
|Human PTPRJ ORF mammalian expression plasmid, C-HA tag||HG13165-CY|
|Human PTPRJ Gene cDNA clone plasmid||HG13165-G|
|Human PTPRJ ORF mammalian expression plasmid, N-Flag tag||HG13165-NF|
|Human PTPRJ ORF mammalian expression plasmid, N-His tag||HG13165-NH|
|Human PTPRJ ORF mammalian expression plasmid, N-Myc tag||HG13165-NM|
|Human PTPRJ ORF mammalian expression plasmid, N-HA tag||HG13165-NY|
|Human PTPRJ natural ORF mammalian expression plasmid||HG13165-UT|
|Learn more about expression Vectors|
DEP1 / PTPRJ (Receptor-type tyrosine-protein phosphatase eta) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. DEP1 / PTPRJ possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. DEP1 / PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. In stable MCF-7 cell lines lines, induction of DEP-1 expression inhibited breast cancer cell growth by 5-10-fold. These data describe PTPs expressed and regulated in breast cancer cell lines during differentiation and identify one PTP, DEP-1, that inhibits the growth of breast cancer cells in vitro.