|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse HMGN3 ORF mammalian expression plasmid, C-GFPSpark tag||MG51434-ACG|
|Mouse HMGN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51434-ACR|
|Mouse HMGN3 ORF mammalian expression plasmid, N-GFPSpark tag||MG51434-ANG|
|Mouse HMGN3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51434-ANR|
|Mouse HMGN3 ORF mammalian expression plasmid, C-Flag tag||MG51434-CF|
|Mouse HMGN3 ORF mammalian expression plasmid, C-His tag||MG51434-CH|
|Mouse HMGN3 ORF mammalian expression plasmid, C-Myc tag||MG51434-CM|
|Mouse HMGN3 ORF mammalian expression plasmid, C-HA tag||MG51434-CY|
|Mouse HMGN3 Gene cDNA clone plasmid||MG51434-G|
|Mouse HMGN3 ORF mammalian expression plasmid, N-Flag tag||MG51434-NF|
|Mouse HMGN3 ORF mammalian expression plasmid, N-His tag||MG51434-NH|
|Mouse HMGN3 ORF mammalian expression plasmid, N-Myc tag||MG51434-NM|
|Mouse HMGN3 ORF mammalian expression plasmid, N-HA tag||MG51434-NY|
|Mouse HMGN3 natural ORF mammalian expression plasmid||MG51434-UT|
|Learn more about expression Vectors|
HMGN3 belongs to the HMGN family and is expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Members of the HMGN family bind to nucleosomes without any specificity for the underlying DNA sequence. They affect the global and local structure of chromatin, as well as the levels of histone modifications and thus play a role in epigenetic regulation of gene expression. HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. It also regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. Both insulin and glucagon levels can be affected by HMGN3. HMGN3 may play a role in ocular development and astrocyte function. It also modulates the expression of pancreatic genes involved in insulin secretion.