|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CTLA4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50503-ACG|
|Mouse CTLA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50503-ACR|
|Mouse CTLA4 ORF mammalian expression plasmid, C-Flag tag||MG50503-CF|
|Mouse CTLA4 ORF mammalian expression plasmid, C-His tag||MG50503-CH|
|Mouse CTLA4 ORF mammalian expression plasmid, C-Myc tag||MG50503-CM|
|Mouse CTLA4 ORF mammalian expression plasmid, C-HA tag||MG50503-CY|
|Mouse CTLA4 Gene cDNA clone plasmid||MG50503-M|
|Mouse CTLA4 ORF mammalian expression plasmid, N-Flag tag||MG50503-NF|
|Mouse CTLA4 ORF mammalian expression plasmid, N-His tag||MG50503-NH|
|Mouse CTLA4 ORF mammalian expression plasmid, N-Myc tag||MG50503-NM|
|Mouse CTLA4 ORF mammalian expression plasmid, N-HA tag||MG50503-NY|
|Mouse CTLA4 natural ORF mammalian expression plasmid||MG50503-UT|
|Learn more about expression Vectors|
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).