|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse USP5 ORF mammalian expression plasmid, C-GFPSpark tag||MG51371-ACG|
|Mouse USP5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51371-ACR|
|Mouse USP5 ORF mammalian expression plasmid, N-GFPSpark tag||MG51371-ANG|
|Mouse USP5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51371-ANR|
|Mouse USP5 ORF mammalian expression plasmid, C-Flag tag||MG51371-CF|
|Mouse USP5 ORF mammalian expression plasmid, C-His tag||MG51371-CH|
|Mouse USP5 ORF mammalian expression plasmid, C-Myc tag||MG51371-CM|
|Mouse USP5 ORF mammalian expression plasmid, C-HA tag||MG51371-CY|
|Mouse USP5 ORF mammalian expression plasmid, N-Flag tag||MG51371-NF|
|Mouse USP5 ORF mammalian expression plasmid, N-His tag||MG51371-NH|
|Mouse USP5 ORF mammalian expression plasmid, N-Myc tag||MG51371-NM|
|Mouse USP5 ORF mammalian expression plasmid, N-HA tag||MG51371-NY|
|Mouse USP5 Gene cDNA clone plasmid||MG51371-U|
|Mouse USP5 natural ORF mammalian expression plasmid||MG51371-UT|
|Learn more about expression Vectors|
Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.