|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CADM3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50476-ACG|
|Mouse CADM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50476-ACR|
|Mouse CADM3 ORF mammalian expression plasmid, C-Flag tag||MG50476-CF|
|Mouse CADM3 ORF mammalian expression plasmid, C-His tag||MG50476-CH|
|Mouse CADM3 ORF mammalian expression plasmid, C-Myc tag||MG50476-CM|
|Mouse CADM3 ORF mammalian expression plasmid, C-HA tag||MG50476-CY|
|Mouse CADM3 Gene cDNA clone plasmid||MG50476-M|
|Mouse CADM3 ORF mammalian expression plasmid, N-Flag tag||MG50476-NF|
|Mouse CADM3 ORF mammalian expression plasmid, N-His tag||MG50476-NH|
|Mouse CADM3 ORF mammalian expression plasmid, N-Myc tag||MG50476-NM|
|Mouse CADM3 ORF mammalian expression plasmid, N-HA tag||MG50476-NY|
|Mouse CADM3 natural ORF mammalian expression plasmid||MG50476-UT|
|Learn more about expression Vectors|
Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 3, also known as Immunoglobulin superfamily member 4B, CADM3, and NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Isoform 1 of CADM3 is expressed mainly in adult and fetal brain. Isoform 2 of CADM3 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 is involved in the cell-cell adhesion. It has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. The interaction with EPB41L1 may regulate structure or function of cell-cell junctions. CADM3 may act as a tumor suppressor in glioma and loss of it in glioma may be caused by histone deacetylation.