|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse JAM3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50465-ACG|
|Mouse JAM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50465-ACR|
|Mouse JAM3 ORF mammalian expression plasmid, C-Flag tag||MG50465-CF|
|Mouse JAM3 ORF mammalian expression plasmid, C-His tag||MG50465-CH|
|Mouse JAM3 ORF mammalian expression plasmid, C-Myc tag||MG50465-CM|
|Mouse JAM3 ORF mammalian expression plasmid, C-HA tag||MG50465-CY|
|Mouse JAM3 Gene cDNA clone plasmid||MG50465-M|
|Mouse JAM3 ORF mammalian expression plasmid, N-Flag tag||MG50465-NF|
|Mouse JAM3 ORF mammalian expression plasmid, N-His tag||MG50465-NH|
|Mouse JAM3 ORF mammalian expression plasmid, N-Myc tag||MG50465-NM|
|Mouse JAM3 ORF mammalian expression plasmid, N-HA tag||MG50465-NY|
|Mouse JAM3 natural ORF mammalian expression plasmid||MG50465-UT|
|Learn more about expression Vectors|
Junctional Adhesion Molecule C Protein & Antibody (JAM-C, JAM3 Protein) also known as Junctional adhesion molecule 3, JAM3, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is an adhesion molecule expressed by endothelial cells (ECs) that plays a role in tight junction formation, leukocyte adhesion, and transendothelial migration. JAM-C is an adhesion molecule that is expressed on cells within the vascular compartment and epithelial cells and, to date, has been largely studied in the context of inflammatory events. JAM-C is also expressed in peripheral nerves and that this expression is localized to Schwann cells at junctions between adjoining myelin end loops. JAM-C is a component of the autotypic junctional attachments of Schwann cells and plays an important role in maintaining the integrity and function of myelinated peripheral nerves. JAM-C was recently shown to be a counter receptor for the leukocyte beta2-integrin Mac-1 (CD11b/CD18), thereby mediating interactions between vascular cells, particularly in inflammatory cell recruitment. JAM-C is up-regulated by oxidized low-density lipoprotein (LDL) and may thereby contribute to increased inflammatory cell recruitment during atherosclerosis. JAM-C may therefore provide a novel molecular target for antagonizing interactions between vascular cells in atherosclerosis. JAM-C was shown to undergo a heterophilic interaction with the leukocyte beta2 integrin Mac-1, thereby mediating interactions between vascular cells in inflammatory cell recruitment. JAM-C undergoes a homophilic interaction via the Arg64-Ile65-Glu66 motif on the membrane-distal Ig domain of the molecule. The homophilic interaction of JAM-C can mediate tumor cell-endothelial cell interactions and may thereby be involved in the process of tumor cell metastasis.