|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse POSTN ORF mammalian expression plasmid, C-GFPSpark tag||MG50450-ACG|
|Mouse POSTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50450-ACR|
|Mouse POSTN ORF mammalian expression plasmid, C-Flag tag||MG50450-CF|
|Mouse POSTN ORF mammalian expression plasmid, C-His tag||MG50450-CH|
|Mouse POSTN ORF mammalian expression plasmid, C-Myc tag||MG50450-CM|
|Mouse POSTN ORF mammalian expression plasmid, C-HA tag||MG50450-CY|
|Mouse POSTN Gene cDNA clone plasmid||MG50450-M|
|Mouse POSTN ORF mammalian expression plasmid, N-Flag tag||MG50450-NF|
|Mouse POSTN ORF mammalian expression plasmid, N-His tag||MG50450-NH|
|Mouse POSTN ORF mammalian expression plasmid, N-Myc tag||MG50450-NM|
|Mouse POSTN ORF mammalian expression plasmid, N-HA tag||MG50450-NY|
|Mouse POSTN natural ORF mammalian expression plasmid||MG50450-UT|
|Learn more about expression Vectors|
Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.