|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MSTN ORF mammalian expression plasmid, C-GFPSpark tag||MG50441-ACG|
|Mouse MSTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50441-ACR|
|Mouse MSTN ORF mammalian expression plasmid, C-Flag tag||MG50441-CF|
|Mouse MSTN ORF mammalian expression plasmid, C-His tag||MG50441-CH|
|Mouse MSTN ORF mammalian expression plasmid, C-Myc tag||MG50441-CM|
|Mouse MSTN ORF mammalian expression plasmid, C-HA tag||MG50441-CY|
|Mouse MSTN Gene cDNA clone plasmid||MG50441-G|
|Mouse MSTN ORF mammalian expression plasmid, N-Flag tag||MG50441-NF|
|Mouse MSTN ORF mammalian expression plasmid, N-His tag||MG50441-NH|
|Mouse MSTN ORF mammalian expression plasmid, N-Myc tag||MG50441-NM|
|Mouse MSTN ORF mammalian expression plasmid, N-HA tag||MG50441-NY|
|Mouse MSTN natural ORF mammalian expression plasmid||MG50441-UT|
|Learn more about expression Vectors|
GDF-8 / Myostatin / MSTN is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF-8 / Myostatin / MSTN is highly expressed in skeletal muscle, and myostatin loss-of-function leads to doubling of skeletal muscle mass. Experiments in mice have improved that GDF-8 / Myostatin / MSTN is a key regulator of mesenchymal stem cell proliferation and differentiation, and mice lacking Myostatin encoding gene show decreased body fat and a generalized increase in bone density and strength. The increase in bone density is observed in most anatomical regions, including the limbs, spine, and jaw, and myostatin inhibitors have been observed to significantly increase bone formation. GDF-8 / Myostatin / MSTN is also expressed in the early phases of fracture healing, and GDF-8 / Myostatin / MSTN deficiency leads to increased fracture callus size and strength. Together, these data suggest that GDF-8 / Myostatin / MSTN has direct effects on the proliferation and differentiation of osteoprogenitor cells, and that GDF-8/Myostatin/MSTN antagonists and inhibitors are likely to enhance both muscle mass and bone strength.