|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51319-ACG|
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51319-ACR|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51319-ANG|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51319-ANR|
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-Flag tag||MG51319-CF|
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-His tag||MG51319-CH|
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-Myc tag||MG51319-CM|
|Mouse ALDH4A1 ORF mammalian expression plasmid, C-HA tag||MG51319-CY|
|Mouse ALDH4A1 Gene cDNA clone plasmid||MG51319-G|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-Flag tag||MG51319-NF|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-His tag||MG51319-NH|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-Myc tag||MG51319-NM|
|Mouse ALDH4A1 ORF mammalian expression plasmid, N-HA tag||MG51319-NY|
|Mouse ALDH4A1 natural ORF mammalian expression plasmid||MG51319-UT|
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ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.