|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse VIM ORF mammalian expression plasmid, C-GFPSpark tag||MG50433-ACG|
|Mouse VIM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50433-ACR|
|Mouse VIM ORF mammalian expression plasmid, N-GFPSpark tag||MG50433-ANG|
|Mouse VIM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50433-ANR|
|Mouse VIM ORF mammalian expression plasmid, C-Flag tag||MG50433-CF|
|Mouse VIM ORF mammalian expression plasmid, C-His tag||MG50433-CH|
|Mouse VIM ORF mammalian expression plasmid, C-Myc tag||MG50433-CM|
|Mouse VIM ORF mammalian expression plasmid, C-HA tag||MG50433-CY|
|Mouse VIM Gene cDNA clone plasmid||MG50433-M|
|Mouse VIM ORF mammalian expression plasmid, N-Flag tag||MG50433-NF|
|Mouse VIM ORF mammalian expression plasmid, N-His tag||MG50433-NH|
|Mouse VIM ORF mammalian expression plasmid, N-Myc tag||MG50433-NM|
|Mouse VIM ORF mammalian expression plasmid, N-HA tag||MG50433-NY|
|Mouse VIM natural ORF mammalian expression plasmid||MG50433-UT|
|Learn more about expression Vectors|
Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.