|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FGF21 ORF mammalian expression plasmid, C-GFPSpark tag||MG50421-ACG|
|Mouse FGF21 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50421-ACR|
|Mouse FGF21 ORF mammalian expression plasmid, C-Flag tag||MG50421-CF|
|Mouse FGF21 ORF mammalian expression plasmid, C-His tag||MG50421-CH|
|Mouse FGF21 ORF mammalian expression plasmid, C-Myc tag||MG50421-CM|
|Mouse FGF21 ORF mammalian expression plasmid, C-HA tag||MG50421-CY|
|Mouse FGF21 ORF mammalian expression plasmid, N-Flag tag||MG50421-NF|
|Mouse FGF21 ORF mammalian expression plasmid, N-His tag||MG50421-NH|
|Mouse FGF21 ORF mammalian expression plasmid, N-Myc tag||MG50421-NM|
|Mouse FGF21 ORF mammalian expression plasmid, N-HA tag||MG50421-NY|
|Mouse FGF21 natural ORF mammalian expression plasmid||MG50421-UT|
|Learn more about expression Vectors|
Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 has a hydrophobic amino terminus, which is a typical signal sequence, and appears to be a secreted protein. The metabolic regulator fibroblast growth factor 21 (FGF21) has antidiabetic properties in animal models of diabetes and obesity. FGF21 is a novel adipokine associated with obesity-related metabolic complications in humans. The paradoxical increase of serum FGF21 in obese individuals, which may be explained by a compensatory response or resistance to FGF21, warrants further investigation. FGF-21, which we have identified as a novel metabolic factor, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.