|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rabbit S100B ORF mammalian expression plasmid, C-GFPSpark tag||TG65082-ACG|
|Rabbit S100B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||TG65082-ACR|
|Rabbit S100B ORF mammalian expression plasmid, N-GFPSpark tag||TG65082-ANG|
|Rabbit S100B ORF mammalian expression plasmid, N-OFPSpark / RFP tag||TG65082-ANR|
|Rabbit S100B ORF mammalian expression plasmid, C-Flag tag||TG65082-CF|
|Rabbit S100B ORF mammalian expression plasmid, C-His tag||TG65082-CH|
|Rabbit S100B ORF mammalian expression plasmid, C-Myc tag||TG65082-CM|
|Rabbit S100B ORF mammalian expression plasmid, C-HA tag||TG65082-CY|
|Rabbit S100B Gene cDNA clone plasmid||TG65082-G|
|Rabbit S100B ORF mammalian expression plasmid, N-Flag tag||TG65082-NF|
|Rabbit S100B ORF mammalian expression plasmid, N-His tag||TG65082-NH|
|Rabbit S100B ORF mammalian expression plasmid, N-Myc tag||TG65082-NM|
|Rabbit S100B ORF mammalian expression plasmid, N-HA tag||TG65082-NY|
|Rabbit S100B natural ORF mammalian expression plasmid||TG65082-UT|
|Learn more about expression Vectors|
S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S100B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.