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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse PLTP ORF mammalian expression plasmid, C-GFPSpark tag||MG52423-ACG|
|Mouse PLTP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52423-ACR|
|Mouse PLTP ORF mammalian expression plasmid, C-Flag tag||MG52423-CF|
|Mouse PLTP ORF mammalian expression plasmid, C-His tag||MG52423-CH|
|Mouse PLTP ORF mammalian expression plasmid, C-Myc tag||MG52423-CM|
|Mouse PLTP ORF mammalian expression plasmid, C-HA tag||MG52423-CY|
|Mouse PLTP Gene cDNA clone plasmid||MG52423-G|
|Mouse PLTP ORF mammalian expression plasmid, N-Flag tag||MG52423-NF|
|Mouse PLTP ORF mammalian expression plasmid, N-His tag||MG52423-NH|
|Mouse PLTP ORF mammalian expression plasmid, N-Myc tag||MG52423-NM|
|Mouse PLTP ORF mammalian expression plasmid, N-HA tag||MG52423-NY|
|Mouse PLTP natural ORF mammalian expression plasmid||MG52423-UT|
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Phospholipid transfer protein, also known as Lipid transfer protein II and PLTP, is a secreted protein which belongs to the BPI/LBP/Plunc superfamily and BPI / LBP family. PLTP is nearly ubiquitously expressed in cells and tissues. PLTP converts HDL into larger and smaller particles. It may play a key role in extracellular phospholipid transport and modulation of hdl particles. High-density lipoproteins (HDL) play a major protective role against the development of coronary artery disease. PLTP is a main factor regulating the size and composition of HDL in the circulation and plays an important role in controlling plasma HDL levels. This is achieved via both the phospholipid transfer activity of PLTP and its capability to cause HDL conversion. PLTP is one of the key lipid transfer proteins in plasma and cerebrospinal fluid. It is involved in novel intracellular functions. PLTP is an important modulator of lipoprotein metabolism, including interparticle phospholipid transfer, remodeling of HDL, cholesterol and phospholipid efflux from peripheral tissues, and the production of hepatic VLDL. PLTP also plays an important role in inflammation and oxidative stress. Accordingly, PLTP has also been implicated in the development of atherosclerosis.