|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CTSA ORF mammalian expression plasmid, C-GFPSpark tag||MG50348-ACG|
|Mouse CTSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50348-ACR|
|Mouse CTSA ORF mammalian expression plasmid, C-Flag tag||MG50348-CF|
|Mouse CTSA ORF mammalian expression plasmid, C-His tag||MG50348-CH|
|Mouse CTSA ORF mammalian expression plasmid, C-Myc tag||MG50348-CM|
|Mouse CTSA ORF mammalian expression plasmid, C-HA tag||MG50348-CY|
|Mouse CTSA Gene cDNA clone plasmid||MG50348-M|
|Mouse CTSA ORF mammalian expression plasmid, N-Flag tag||MG50348-NF|
|Mouse CTSA ORF mammalian expression plasmid, N-His tag||MG50348-NH|
|Mouse CTSA ORF mammalian expression plasmid, N-Myc tag||MG50348-NM|
|Mouse CTSA ORF mammalian expression plasmid, N-HA tag||MG50348-NY|
|Mouse CTSA natural ORF mammalian expression plasmid||MG50348-UT|
|Learn more about expression Vectors|
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.