|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rabbit ERBB3 ORF mammalian expression plasmid, C-GFPSpark tag||TG65001-ACG|
|Rabbit ERBB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||TG65001-ACR|
|Rabbit ERBB3 ORF mammalian expression plasmid, C-Flag tag||TG65001-CF|
|Rabbit ERBB3 ORF mammalian expression plasmid, C-His tag||TG65001-CH|
|Rabbit ERBB3 ORF mammalian expression plasmid, C-Myc tag||TG65001-CM|
|Rabbit ERBB3 ORF mammalian expression plasmid, C-HA tag||TG65001-CY|
|Rabbit ERBB3 Gene cDNA clone plasmid||TG65001-G|
|Rabbit ERBB3 ORF mammalian expression plasmid, N-Flag tag||TG65001-NF|
|Rabbit ERBB3 ORF mammalian expression plasmid, N-His tag||TG65001-NH|
|Rabbit ERBB3 ORF mammalian expression plasmid, N-Myc tag||TG65001-NM|
|Rabbit ERBB3 ORF mammalian expression plasmid, N-HA tag||TG65001-NY|
|Rabbit ERBB3 natural ORF mammalian expression plasmid||TG65001-UT|
|Learn more about expression Vectors|
ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.