|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TPM4 ORF mammalian expression plasmid, C-GFPSpark tag||RG81616-ACG|
|Rat TPM4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81616-ACR|
|Rat TPM4 ORF mammalian expression plasmid, C-Flag tag||RG81616-CF|
|Rat TPM4 ORF mammalian expression plasmid, C-His tag||RG81616-CH|
|Rat TPM4 ORF mammalian expression plasmid, C-Myc tag||RG81616-CM|
|Rat TPM4 ORF mammalian expression plasmid, C-HA tag||RG81616-CY|
|Rat TPM4 Gene cDNA clone plasmid||RG81616-G|
|Rat TPM4 ORF mammalian expression plasmid, N-Flag tag||RG81616-NF|
|Rat TPM4 ORF mammalian expression plasmid, N-His tag||RG81616-NH|
|Rat TPM4 ORF mammalian expression plasmid, N-Myc tag||RG81616-NM|
|Rat TPM4 ORF mammalian expression plasmid, N-HA tag||RG81616-NY|
|Rat TPM4 natural ORF mammalian expression plasmid||RG81616-UT|
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TPM4, also known as tropomyosin 4, is a member of the tropomyosin family. Members of this family are actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. TPM4 is expressed in cardiac tissue and platelets. It is highly expressed in the platelets of hypertensive patients. TPM4 plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells it is implicated in stabilizing cytoskeleton actin filaments.