|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat VRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81611-ACG|
|Rat VRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81611-ACR|
|Rat VRK1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81611-ANG|
|Rat VRK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81611-ANR|
|Rat VRK1 ORF mammalian expression plasmid, C-Flag tag||RG81611-CF|
|Rat VRK1 ORF mammalian expression plasmid, C-His tag||RG81611-CH|
|Rat VRK1 ORF mammalian expression plasmid, C-Myc tag||RG81611-CM|
|Rat VRK1 ORF mammalian expression plasmid, C-HA tag||RG81611-CY|
|Rat VRK1 Gene cDNA clone plasmid||RG81611-G|
|Rat VRK1 ORF mammalian expression plasmid, N-Flag tag||RG81611-NF|
|Rat VRK1 ORF mammalian expression plasmid, N-His tag||RG81611-NH|
|Rat VRK1 ORF mammalian expression plasmid, N-Myc tag||RG81611-NM|
|Rat VRK1 ORF mammalian expression plasmid, N-HA tag||RG81611-NY|
|Rat VRK1 natural ORF mammalian expression plasmid||RG81611-UT|
|Learn more about expression Vectors|
VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.