|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PDHA1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51206-ACG|
|Mouse PDHA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51206-ACR|
|Mouse PDHA1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51206-ANG|
|Mouse PDHA1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51206-ANR|
|Mouse PDHA1 ORF mammalian expression plasmid, C-Flag tag||MG51206-CF|
|Mouse PDHA1 ORF mammalian expression plasmid, C-His tag||MG51206-CH|
|Mouse PDHA1 ORF mammalian expression plasmid, C-Myc tag||MG51206-CM|
|Mouse PDHA1 ORF mammalian expression plasmid, C-HA tag||MG51206-CY|
|Mouse PDHA1 ORF mammalian expression plasmid, N-Flag tag||MG51206-NF|
|Mouse PDHA1 ORF mammalian expression plasmid, N-His tag||MG51206-NH|
|Mouse PDHA1 ORF mammalian expression plasmid, N-Myc tag||MG51206-NM|
|Mouse PDHA1 ORF mammalian expression plasmid, N-HA tag||MG51206-NY|
|Mouse PDHA1 Gene cDNA clone plasmid||MG51206-U|
|Mouse PDHA1 natural ORF mammalian expression plasmid||MG51206-UT|
|Learn more about expression Vectors|
PDHA1, also known as C54G1, is an alpha subunit of pyruvate dehydrogenase. Pyruvate dehydrogenase, together with dihydrolipoamide acetyltransferase and lipoamide dehydrogenase, composes the pyruvate dehydrogenase (PDH) complex. The PDH complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. PDHA1 plays a key role in the function of the PDH complex. Defects in PDHA1 can cause pyruvate dehydrogenase E1-alpha deficiency. Defects in PDHA1 also are the cause of X-linked Leigh syndrome (X-LS). X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord.