|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TrkC ORF mammalian expression plasmid, C-GFPSpark tag||MG50320-ACG|
|Mouse TrkC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50320-ACR|
|Mouse TrkC ORF mammalian expression plasmid, C-Flag tag||MG50320-CF|
|Mouse TrkC ORF mammalian expression plasmid, C-His tag||MG50320-CH|
|Mouse TrkC ORF mammalian expression plasmid, C-Myc tag||MG50320-CM|
|Mouse TrkC ORF mammalian expression plasmid, C-HA tag||MG50320-CY|
|Mouse TrkC Gene cDNA clone plasmid||MG50320-G|
|Mouse TrkC ORF mammalian expression plasmid, N-Flag tag||MG50320-NF|
|Mouse TrkC ORF mammalian expression plasmid, N-His tag||MG50320-NH|
|Mouse TrkC ORF mammalian expression plasmid, N-Myc tag||MG50320-NM|
|Mouse TrkC ORF mammalian expression plasmid, N-HA tag||MG50320-NY|
|Mouse TrkC natural ORF mammalian expression plasmid||MG50320-UT|
|Learn more about expression Vectors|
NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.