|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat FTH1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81341-ACG|
|Rat FTH1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81341-ACR|
|Rat FTH1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81341-ANG|
|Rat FTH1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81341-ANR|
|Rat FTH1 ORF mammalian expression plasmid, C-Flag tag||RG81341-CF|
|Rat FTH1 ORF mammalian expression plasmid, C-His tag||RG81341-CH|
|Rat FTH1 ORF mammalian expression plasmid, C-Myc tag||RG81341-CM|
|Rat FTH1 ORF mammalian expression plasmid, C-HA tag||RG81341-CY|
|Rat FTH1 Gene cDNA clone plasmid||RG81341-G|
|Rat FTH1 ORF mammalian expression plasmid, N-Flag tag||RG81341-NF|
|Rat FTH1 ORF mammalian expression plasmid, N-His tag||RG81341-NH|
|Rat FTH1 ORF mammalian expression plasmid, N-Myc tag||RG81341-NM|
|Rat FTH1 ORF mammalian expression plasmid, N-HA tag||RG81341-NY|
|Rat FTH1 natural ORF mammalian expression plasmid||RG81341-UT|
|Learn more about expression Vectors|
FTH1 (ferritin, heavy polypeptide 1) is the heavy subunit of ferritin which is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. FTH1 gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. FTH1 stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. It has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. It also plays a role in delivery of iron to cells. FTH1 mediates iron uptake in capsule cells of the developing kidney.