|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PTGDS ORF mammalian expression plasmid, C-GFPSpark tag||RG81340-ACG|
|Rat PTGDS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81340-ACR|
|Rat PTGDS ORF mammalian expression plasmid, C-Flag tag||RG81340-CF|
|Rat PTGDS ORF mammalian expression plasmid, C-His tag||RG81340-CH|
|Rat PTGDS ORF mammalian expression plasmid, C-Myc tag||RG81340-CM|
|Rat PTGDS ORF mammalian expression plasmid, C-HA tag||RG81340-CY|
|Rat PTGDS Gene cDNA clone plasmid||RG81340-G|
|Rat PTGDS ORF mammalian expression plasmid, N-Flag tag||RG81340-NF|
|Rat PTGDS ORF mammalian expression plasmid, N-His tag||RG81340-NH|
|Rat PTGDS ORF mammalian expression plasmid, N-Myc tag||RG81340-NM|
|Rat PTGDS ORF mammalian expression plasmid, N-HA tag||RG81340-NY|
|Rat PTGDS natural ORF mammalian expression plasmid||RG81340-UT|
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PTGDS, also known as L-PGDS, belongs to the calycin superfamily，lipocalin family. Lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in smooth muscle contraction/relaxation and a variety of central nervous system functions. PTGDS may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.