|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MAP2K1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50312-ACG|
|Mouse MAP2K1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50312-ACR|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50312-ANG|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50312-ANR|
|Mouse MAP2K1 ORF mammalian expression plasmid, C-Flag tag||MG50312-CF|
|Mouse MAP2K1 ORF mammalian expression plasmid, C-His tag||MG50312-CH|
|Mouse MAP2K1 ORF mammalian expression plasmid, C-Myc tag||MG50312-CM|
|Mouse MAP2K1 ORF mammalian expression plasmid, C-HA tag||MG50312-CY|
|Mouse MAP2K1 Gene cDNA clone plasmid||MG50312-M|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-Flag tag||MG50312-NF|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-His tag||MG50312-NH|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-Myc tag||MG50312-NM|
|Mouse MAP2K1 ORF mammalian expression plasmid, N-HA tag||MG50312-NY|
|Mouse MAP2K1 natural ORF mammalian expression plasmid||MG50312-UT|
|Learn more about expression Vectors|
MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.