|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human IFITM3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14141-ACG|
|Human IFITM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14141-ACR|
|Human IFITM3 ORF mammalian expression plasmid, C-Flag tag||HG14141-CF|
|Human IFITM3 ORF mammalian expression plasmid, C-His tag||HG14141-CH|
|Human IFITM3 ORF mammalian expression plasmid, C-Myc tag||HG14141-CM|
|Human IFITM3 ORF mammalian expression plasmid, C-HA tag||HG14141-CY|
|Human IFITM3 Gene cDNA clone plasmid||HG14141-G|
|Human IFITM3 ORF mammalian expression plasmid, N-Flag tag||HG14141-NF|
|Human IFITM3 ORF mammalian expression plasmid, N-His tag||HG14141-NH|
|Human IFITM3 ORF mammalian expression plasmid, N-Myc tag||HG14141-NM|
|Human IFITM3 ORF mammalian expression plasmid, N-HA tag||HG14141-NY|
|Human IFITM3 natural ORF mammalian expression plasmid||HG14141-UT|
|Learn more about expression Vectors|
Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. IFITM3 is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. It is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. Viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.