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Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Flag tag

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Mouse SRC cDNA Clone Product Information
Gene_bank_ref_id:NM_009271.3
RefSeq ORF Size:1626bp
cDNA Description:Full length Clone DNA of Mus musculus Rous sarcoma oncogene transcript variant 1 with C terminal Flag tag.
Gene Synonym:AW259666, pp60c-src, Src
Species:Mouse
Vector:pCMV3-C-FLAG
Plasmid:
Restriction Site:
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Flag tag on other vectors
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-GFPSpark tagMG51118-ACG$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-OFPSpark / RFP tagMG51118-ACR$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-GFPSpark tagMG51118-ANG$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-OFPSpark / RFP tagMG51118-ANR$345
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Flag tagMG51118-CF$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-His tagMG51118-CH$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-Myc tagMG51118-CM$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, C-HA tagMG51118-CY$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 Gene cDNA clone plasmidMG51118-G$195
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, Flag tagMG51118-G-F$395
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-Flag tagMG51118-NF$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-His tagMG51118-NH$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-Myc tagMG51118-NM$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 ORF mammalian expression plasmid, N-HA tagMG51118-NY$315
Mouse SRC Kinase / Proto-oncogene c-Src transcript variant 1 natural ORF mammalian expression plasmidMG51118-UT$315
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Background

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

References
  • Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
  • Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
  • Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
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    Catalog: MG51118-CF
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