|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse C1QB ORF mammalian expression plasmid, C-GFPSpark tag||MG50295-ACG|
|Mouse C1QB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50295-ACR|
|Mouse C1QB ORF mammalian expression plasmid, C-Flag tag||MG50295-CF|
|Mouse C1QB ORF mammalian expression plasmid, C-His tag||MG50295-CH|
|Mouse C1QB ORF mammalian expression plasmid, C-Myc tag||MG50295-CM|
|Mouse C1QB ORF mammalian expression plasmid, C-HA tag||MG50295-CY|
|Mouse C1QB Gene cDNA clone plasmid||MG50295-M|
|Mouse C1QB ORF mammalian expression plasmid, N-Flag tag||MG50295-NF|
|Mouse C1QB ORF mammalian expression plasmid, N-His tag||MG50295-NH|
|Mouse C1QB ORF mammalian expression plasmid, N-Myc tag||MG50295-NM|
|Mouse C1QB ORF mammalian expression plasmid, N-HA tag||MG50295-NY|
|Mouse C1QB natural ORF mammalian expression plasmid||MG50295-UT|
|Learn more about expression Vectors|
Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge.