|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ESM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51108-ACG|
|Mouse ESM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51108-ACR|
|Mouse ESM1 ORF mammalian expression plasmid, C-Flag tag||MG51108-CF|
|Mouse ESM1 ORF mammalian expression plasmid, C-His tag||MG51108-CH|
|Mouse ESM1 ORF mammalian expression plasmid, C-Myc tag||MG51108-CM|
|Mouse ESM1 ORF mammalian expression plasmid, C-HA tag||MG51108-CY|
|Mouse ESM1 Gene cDNA clone plasmid||MG51108-G|
|Mouse ESM1 ORF mammalian expression plasmid, N-Flag tag||MG51108-NF|
|Mouse ESM1 ORF mammalian expression plasmid, N-His tag||MG51108-NH|
|Mouse ESM1 ORF mammalian expression plasmid, N-Myc tag||MG51108-NM|
|Mouse ESM1 ORF mammalian expression plasmid, N-HA tag||MG51108-NY|
|Mouse ESM1 natural ORF mammalian expression plasmid||MG51108-UT|
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ESM1 is a secreted protein which is produced by adipocytes. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. ESM1 is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Recently, ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.