|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse PTMA ORF mammalian expression plasmid, C-GFPSpark tag||MG50272-ACG|
|Mouse PTMA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50272-ACR|
|Mouse PTMA ORF mammalian expression plasmid, N-GFPSpark tag||MG50272-ANG|
|Mouse PTMA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50272-ANR|
|Mouse PTMA ORF mammalian expression plasmid, C-Flag tag||MG50272-CF|
|Mouse PTMA ORF mammalian expression plasmid, C-His tag||MG50272-CH|
|Mouse PTMA ORF mammalian expression plasmid, C-Myc tag||MG50272-CM|
|Mouse PTMA ORF mammalian expression plasmid, C-HA tag||MG50272-CY|
|Mouse PTMA Gene cDNA clone plasmid||MG50272-M|
|Mouse PTMA ORF mammalian expression plasmid, N-Flag tag||MG50272-NF|
|Mouse PTMA ORF mammalian expression plasmid, N-His tag||MG50272-NH|
|Mouse PTMA ORF mammalian expression plasmid, N-Myc tag||MG50272-NM|
|Mouse PTMA ORF mammalian expression plasmid, N-HA tag||MG50272-NY|
|Mouse PTMA natural ORF mammalian expression plasmid||MG50272-UT|
|Learn more about expression Vectors|
PTMA (prothymosin, alpha, N-GST chimera) is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin a1. Thymosins are named becaues they were originally isolated from the thymus. But now in many other tissues, thymosins also can be detected. Thymosins have diverse biological activities, and two in particular, thymosins a1 and _4, have potentially important uses in medicine, some of which have already progressed from the laboratory to the clinic. In general, PTMA is associated with cellular proliferation and carcinogenesis (Eschenfeldt et al., 1986), cellular and viral transcription (Cotter et al., 2000), protection against apoptosis and chromatin remodelling (Karetsou et al., 1998). PTMA may have a dual role both intracellulary and extracellulary. In relation to diseases, thymosins have been categorized as biological response modifiers. Thymosin a1 is derived from PTMA. For animals that lack thymus glands, thymosin a1 is responsible for the activity of that preparation in restoring immune function.