|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SULT1A1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81238-ACG|
|Rat SULT1A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81238-ACR|
|Rat SULT1A1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81238-ANG|
|Rat SULT1A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81238-ANR|
|Rat SULT1A1 ORF mammalian expression plasmid, C-Flag tag||RG81238-CF|
|Rat SULT1A1 ORF mammalian expression plasmid, C-His tag||RG81238-CH|
|Rat SULT1A1 ORF mammalian expression plasmid, C-Myc tag||RG81238-CM|
|Rat SULT1A1 ORF mammalian expression plasmid, C-HA tag||RG81238-CY|
|Rat SULT1A1 ORF mammalian expression plasmid, N-Flag tag||RG81238-NF|
|Rat SULT1A1 ORF mammalian expression plasmid, N-His tag||RG81238-NH|
|Rat SULT1A1 ORF mammalian expression plasmid, N-Myc tag||RG81238-NM|
|Rat SULT1A1 ORF mammalian expression plasmid, N-HA tag||RG81238-NY|
|Rat SULT1A1 Gene cDNA clone plasmid||RG81238-U|
|Rat SULT1A1 natural ORF mammalian expression plasmid||RG81238-UT|
|Learn more about expression Vectors|
Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 10 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer.