|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse GP1bb ORF mammalian expression plasmid, C-GFPSpark tag||MG51075-ACG|
|Mouse GP1bb ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51075-ACR|
|Mouse GP1bb ORF mammalian expression plasmid, C-Flag tag||MG51075-CF|
|Mouse GP1bb ORF mammalian expression plasmid, C-His tag||MG51075-CH|
|Mouse GP1bb ORF mammalian expression plasmid, C-Myc tag||MG51075-CM|
|Mouse GP1bb ORF mammalian expression plasmid, C-HA tag||MG51075-CY|
|Mouse GP1bb Gene cDNA clone plasmid||MG51075-G|
|Mouse GP1bb ORF mammalian expression plasmid, N-Flag tag||MG51075-NF|
|Mouse GP1bb ORF mammalian expression plasmid, N-His tag||MG51075-NH|
|Mouse GP1bb ORF mammalian expression plasmid, N-Myc tag||MG51075-NM|
|Mouse GP1bb ORF mammalian expression plasmid, N-HA tag||MG51075-NY|
|Mouse GP1bb natural ORF mammalian expression plasmid||MG51075-UT|
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Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and in thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and noncovalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta and CD42c, is single-pass type I membrane protein expressed in heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF), and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.