|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse FLT3L ORF mammalian expression plasmid, C-GFPSpark tag||MG51113-ACG|
|Mouse FLT3L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51113-ACR|
|Mouse FLT3L ORF mammalian expression plasmid, C-Flag tag||MG51113-CF|
|Mouse FLT3L ORF mammalian expression plasmid, C-His tag||MG51113-CH|
|Mouse FLT3L ORF mammalian expression plasmid, C-Myc tag||MG51113-CM|
|Mouse FLT3L ORF mammalian expression plasmid, C-HA tag||MG51113-CY|
|Mouse FLT3L Gene cDNA clone plasmid||MG51113-G|
|Mouse FLT3L ORF mammalian expression plasmid, N-Flag tag||MG51113-NF|
|Mouse FLT3L ORF mammalian expression plasmid, N-His tag||MG51113-NH|
|Mouse FLT3L ORF mammalian expression plasmid, N-Myc tag||MG51113-NM|
|Mouse FLT3L ORF mammalian expression plasmid, N-HA tag||MG51113-NY|
|Mouse FLT3L natural ORF mammalian expression plasmid||MG51113-UT|
|Learn more about expression Vectors|
FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.