|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SDF2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50241-ACG|
|Mouse SDF2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50241-ACR|
|Mouse SDF2 ORF mammalian expression plasmid, C-Flag tag||MG50241-CF|
|Mouse SDF2 ORF mammalian expression plasmid, C-His tag||MG50241-CH|
|Mouse SDF2 ORF mammalian expression plasmid, C-Myc tag||MG50241-CM|
|Mouse SDF2 ORF mammalian expression plasmid, C-HA tag||MG50241-CY|
|Mouse SDF2 Gene cDNA clone plasmid||MG50241-M|
|Mouse SDF2 ORF mammalian expression plasmid, N-Flag tag||MG50241-NF|
|Mouse SDF2 ORF mammalian expression plasmid, N-His tag||MG50241-NH|
|Mouse SDF2 ORF mammalian expression plasmid, N-Myc tag||MG50241-NM|
|Mouse SDF2 ORF mammalian expression plasmid, N-HA tag||MG50241-NY|
|Mouse SDF2 natural ORF mammalian expression plasmid||MG50241-UT|
|Learn more about expression Vectors|
Stromal derived factors (SDFs) are a loosely defined group of molecules that are generated by stromal cells. Two of the stromal derived factors, SDF-1 and SDF-4 belong to the chemokine family. Other SDFs, such as SDF-2 and SDF-5 are not well defined and their biological functions are less known. SDF-2 is first isolated from the mouse stromal cell line ST2 as a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92 %, and the aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. SDF-1 and its receptor are strongly indicated in the progression of various cancers including breast cancer. SDF-2, SDF2-L1, SDF-4, and SDF-5 are ubiquitously expressed in various cancer cell lines and SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues. These SDFs have prognostic value and warrant further investigation in their biological functions and clinical value.