|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat METAP2 ORF mammalian expression plasmid, C-GFPSpark tag||RG81187-ACG|
|Rat METAP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81187-ACR|
|Rat METAP2 ORF mammalian expression plasmid, N-GFPSpark tag||RG81187-ANG|
|Rat METAP2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81187-ANR|
|Rat METAP2 ORF mammalian expression plasmid, C-Flag tag||RG81187-CF|
|Rat METAP2 ORF mammalian expression plasmid, C-His tag||RG81187-CH|
|Rat METAP2 ORF mammalian expression plasmid, C-Myc tag||RG81187-CM|
|Rat METAP2 ORF mammalian expression plasmid, C-HA tag||RG81187-CY|
|Rat METAP2 ORF mammalian expression plasmid, N-Flag tag||RG81187-NF|
|Rat METAP2 ORF mammalian expression plasmid, N-His tag||RG81187-NH|
|Rat METAP2 ORF mammalian expression plasmid, N-Myc tag||RG81187-NM|
|Rat METAP2 ORF mammalian expression plasmid, N-HA tag||RG81187-NY|
|Rat METAP2 Gene cDNA clone plasmid||RG81187-U|
|Rat METAP2 natural ORF mammalian expression plasmid||RG81187-UT|
|Learn more about expression Vectors|
METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.