|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SHH ORF mammalian expression plasmid, C-GFPSpark tag||MG51100-ACG|
|Mouse SHH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51100-ACR|
|Mouse SHH ORF mammalian expression plasmid, C-Flag tag||MG51100-CF|
|Mouse SHH ORF mammalian expression plasmid, C-His tag||MG51100-CH|
|Mouse SHH ORF mammalian expression plasmid, C-Myc tag||MG51100-CM|
|Mouse SHH ORF mammalian expression plasmid, C-HA tag||MG51100-CY|
|Mouse SHH Gene cDNA clone plasmid||MG51100-G|
|Mouse SHH ORF mammalian expression plasmid, N-Flag tag||MG51100-NF|
|Mouse SHH ORF mammalian expression plasmid, N-His tag||MG51100-NH|
|Mouse SHH ORF mammalian expression plasmid, N-Myc tag||MG51100-NM|
|Mouse SHH ORF mammalian expression plasmid, N-HA tag||MG51100-NY|
|Mouse SHH natural ORF mammalian expression plasmid||MG51100-UT|
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Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.