|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MOG ORF mammalian expression plasmid, C-GFPSpark tag||MG51046-ACG|
|Mouse MOG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51046-ACR|
|Mouse MOG ORF mammalian expression plasmid, C-Flag tag||MG51046-CF|
|Mouse MOG ORF mammalian expression plasmid, C-His tag||MG51046-CH|
|Mouse MOG ORF mammalian expression plasmid, C-Myc tag||MG51046-CM|
|Mouse MOG ORF mammalian expression plasmid, C-HA tag||MG51046-CY|
|Mouse MOG Gene cDNA clone plasmid||MG51046-G|
|Mouse MOG ORF mammalian expression plasmid, N-Flag tag||MG51046-NF|
|Mouse MOG ORF mammalian expression plasmid, N-His tag||MG51046-NH|
|Mouse MOG ORF mammalian expression plasmid, N-Myc tag||MG51046-NM|
|Mouse MOG ORF mammalian expression plasmid, N-HA tag||MG51046-NY|
|Mouse MOG natural ORF mammalian expression plasmid||MG51046-UT|
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Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to immunoglobulin superfamily, and contains an Ig-like domain followed by two potential membrane-spanning regions. MOG is expressed only in the CNS with very low content (approximately 0.1% total proteins) in oligodendrogliocyte membrane. Three possible functions for MOG were suggested: (a) a cellular adhesive molecule, (b) a regulator of oligodendrocyte microtubule stability, and (c) a mediator of interactions between myelin and the immune system, in particular, the complement cascade. A direct interaction might exist between the membrane-associated regions of MOG and the myelin-specific glycolipid galactocerebroside (Gal-C), and such an interaction may have important consequences regarding the membrane topology and function of both molecules. It is considered that MOG is an autoantigen capable to produce a demyelinating multiple sclerosis-like disease in experimental animals.