|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse IL4 ORF mammalian expression plasmid, C-GFPSpark tag||MG51084-ACG|
|Mouse IL4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51084-ACR|
|Mouse IL4 ORF mammalian expression plasmid, C-Flag tag||MG51084-CF|
|Mouse IL4 ORF mammalian expression plasmid, C-His tag||MG51084-CH|
|Mouse IL4 ORF mammalian expression plasmid, C-Myc tag||MG51084-CM|
|Mouse IL4 ORF mammalian expression plasmid, C-HA tag||MG51084-CY|
|Mouse IL4 Gene cDNA clone plasmid||MG51084-G|
|Mouse IL4 ORF mammalian expression plasmid, N-Flag tag||MG51084-NF|
|Mouse IL4 ORF mammalian expression plasmid, N-His tag||MG51084-NH|
|Mouse IL4 ORF mammalian expression plasmid, N-Myc tag||MG51084-NM|
|Mouse IL4 ORF mammalian expression plasmid, N-HA tag||MG51084-NY|
|Mouse IL4 natural ORF mammalian expression plasmid||MG51084-UT|
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Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.