|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CRTAM ORF mammalian expression plasmid, C-GFPSpark tag||HG11975-ACG|
|Human CRTAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11975-ACR|
|Human CRTAM ORF mammalian expression plasmid, C-Flag tag||HG11975-CF|
|Human CRTAM ORF mammalian expression plasmid, C-His tag||HG11975-CH|
|Human CRTAM ORF mammalian expression plasmid, C-Myc tag||HG11975-CM|
|Human CRTAM ORF mammalian expression plasmid, C-HA tag||HG11975-CY|
|Human CRTAM Gene cDNA clone plasmid||HG11975-G|
|Human CRTAM ORF mammalian expression plasmid, Flag tag||HG11975-G-F|
|Human CRTAM ORF mammalian expression plasmid, N-Flag tag||HG11975-NF|
|Human CRTAM ORF mammalian expression plasmid, N-His tag||HG11975-NH|
|Human CRTAM ORF mammalian expression plasmid, N-Myc tag||HG11975-NM|
|Human CRTAM ORF mammalian expression plasmid, N-HA tag||HG11975-NY|
|Human CRTAM natural ORF mammalian expression plasmid||HG11975-UT|
|Learn more about expression Vectors|
Cytotoxic and regulatory T-cell molecule, also known as Class-I MHC-restricted T-cell-associated molecule and CRTAM, is a single-pass type I membrane protein which belongs to the nectin family. CRTAM contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. It is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in purkinje neurons in cerebellum. It is expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. CRTAM is a member of the immunoglobulin superfamily that complies with the structural characteristics of the JAM family of proteins and is phylogenetically more closely related to nectin-like proteins. It is a molecule involved in epithelial cell adhesion. CRTAM is sensitive to intermediate filament disruption and treatment of monolayers with soluble CRTAM enhances cell-cell dissociation and lowers transepithelial electrical resistance. CRTAM may also induce retention by binding to CD8+ dendritic cells (DCs) at the late stage of activation before proliferation.