|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SMYD3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50930-ACG|
|Mouse SMYD3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50930-ACR|
|Mouse SMYD3 ORF mammalian expression plasmid, N-GFPSpark tag||MG50930-ANG|
|Mouse SMYD3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50930-ANR|
|Mouse SMYD3 ORF mammalian expression plasmid, C-Flag tag||MG50930-CF|
|Mouse SMYD3 ORF mammalian expression plasmid, C-His tag||MG50930-CH|
|Mouse SMYD3 ORF mammalian expression plasmid, C-Myc tag||MG50930-CM|
|Mouse SMYD3 ORF mammalian expression plasmid, C-HA tag||MG50930-CY|
|Mouse SMYD3 Gene cDNA clone plasmid||MG50930-G|
|Mouse SMYD3 ORF mammalian expression plasmid, N-Flag tag||MG50930-NF|
|Mouse SMYD3 ORF mammalian expression plasmid, N-His tag||MG50930-NH|
|Mouse SMYD3 ORF mammalian expression plasmid, N-Myc tag||MG50930-NM|
|Mouse SMYD3 ORF mammalian expression plasmid, N-HA tag||MG50930-NY|
|Mouse SMYD3 natural ORF mammalian expression plasmid||MG50930-UT|
|Learn more about expression Vectors|
SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.