|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse KLK-7 ORF mammalian expression plasmid, C-GFPSpark tag||MG50921-ACG|
|Mouse KLK-7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50921-ACR|
|Mouse KLK-7 ORF mammalian expression plasmid, C-Flag tag||MG50921-CF|
|Mouse KLK-7 ORF mammalian expression plasmid, C-His tag||MG50921-CH|
|Mouse KLK-7 ORF mammalian expression plasmid, C-Myc tag||MG50921-CM|
|Mouse KLK-7 ORF mammalian expression plasmid, C-HA tag||MG50921-CY|
|Mouse KLK-7 Gene cDNA clone plasmid||MG50921-G|
|Mouse KLK-7 ORF mammalian expression plasmid, N-Flag tag||MG50921-NF|
|Mouse KLK-7 ORF mammalian expression plasmid, N-His tag||MG50921-NH|
|Mouse KLK-7 ORF mammalian expression plasmid, N-Myc tag||MG50921-NM|
|Mouse KLK-7 ORF mammalian expression plasmid, N-HA tag||MG50921-NY|
|Mouse KLK-7 natural ORF mammalian expression plasmid||MG50921-UT|
|Learn more about expression Vectors|
Kallikrein-7, also known as kallikrein-related peptidase 7, Stratum corneum chymotryptic enzyme, Serine protease 6, KLK7, and PRSS6, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. Members of the Kallikrein family are involved in various malignancies such as prostate (PSA, KLK2, KLK15), ovarian (KLK4, KLK5, KLK6, KLK8, KLK10), and breast cancer (KLK10, KLK13, KLK14). Kallikrein-7 / KLK7 appears to be increased in ovarian cancer and higher KLK7 expression in ovarian cancer tissue is associated with poorer prognosis of ovarian cancer patients. Kallikrein-7 / KLK7 is abundantly expressed in the skin and is expressed by keratinocytes in the epidermis. Kallikrein-7 / KLK7 is up-regulated in ovarian carcinoma, especially late-stage serous carcinoma, compared with normal ovaries and benign adenomas (at the protein level). It was significantly associated with shorter overall survival (OS) and disease-free survival (DFS). Kallikrein-7 / KLK7 may catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. KLK7 also plays a role in the activation of precursors to inflammatory cytokines.