|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ST6GAL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80866-ACG|
|Rat ST6GAL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80866-ACR|
|Rat ST6GAL1 ORF mammalian expression plasmid, C-Flag tag||RG80866-CF|
|Rat ST6GAL1 ORF mammalian expression plasmid, C-His tag||RG80866-CH|
|Rat ST6GAL1 ORF mammalian expression plasmid, C-Myc tag||RG80866-CM|
|Rat ST6GAL1 ORF mammalian expression plasmid, C-HA tag||RG80866-CY|
|Rat ST6GAL1 ORF mammalian expression plasmid, N-Flag tag||RG80866-NF|
|Rat ST6GAL1 ORF mammalian expression plasmid, N-His tag||RG80866-NH|
|Rat ST6GAL1 ORF mammalian expression plasmid, N-Myc tag||RG80866-NM|
|Rat ST6GAL1 ORF mammalian expression plasmid, N-HA tag||RG80866-NY|
|Rat ST6GAL1 Gene cDNA clone plasmid||RG80866-U|
|Rat ST6GAL1 natural ORF mammalian expression plasmid||RG80866-UT|
|Learn more about expression Vectors|
Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.