|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PDGFD ORF mammalian expression plasmid, C-GFPSpark tag||MG50912-ACG|
|Mouse PDGFD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50912-ACR|
|Mouse PDGFD ORF mammalian expression plasmid, C-Flag tag||MG50912-CF|
|Mouse PDGFD ORF mammalian expression plasmid, C-His tag||MG50912-CH|
|Mouse PDGFD ORF mammalian expression plasmid, C-Myc tag||MG50912-CM|
|Mouse PDGFD ORF mammalian expression plasmid, C-HA tag||MG50912-CY|
|Mouse PDGFD Gene cDNA clone plasmid||MG50912-G|
|Mouse PDGFD ORF mammalian expression plasmid, N-Flag tag||MG50912-NF|
|Mouse PDGFD ORF mammalian expression plasmid, N-His tag||MG50912-NH|
|Mouse PDGFD ORF mammalian expression plasmid, N-Myc tag||MG50912-NM|
|Mouse PDGFD ORF mammalian expression plasmid, N-HA tag||MG50912-NY|
|Mouse PDGFD natural ORF mammalian expression plasmid||MG50912-UT|
|Learn more about expression Vectors|
Platelet-derived growth factor D (PDGF-D), also known as Iris-expressed growth factor, is a member of the PDGF/vascular endothelial growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. PDGF-D/PDGFD only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. The expression of PDGF-D/PDGFD in the eye is tissue-specific. In the anterior segment, it is localized to iris and ciliary body, whereas in the retina, PDGF-D/PDGFD is restricted to the outer plexiform layer. PDGF-D/PDGFD is present in aqueous humor but is not detectable in mature lens or in mouse lens-derived alphaTN4-1 cells. PDGF-D/PDGFD is highly expressed in human breast cancer and facilitates tumor growth and lymph node metastasis, making it a potential target in breast cancer. PDGF-D/PDGFD increases drug delivery and hence improves the efficacy of chemotherapy through vessel normalization. Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract.