|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PLAT ORF mammalian expression plasmid, C-GFPSpark tag||MG50910-ACG|
|Mouse PLAT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50910-ACR|
|Mouse PLAT ORF mammalian expression plasmid, C-Flag tag||MG50910-CF|
|Mouse PLAT ORF mammalian expression plasmid, C-His tag||MG50910-CH|
|Mouse PLAT ORF mammalian expression plasmid, C-Myc tag||MG50910-CM|
|Mouse PLAT ORF mammalian expression plasmid, C-HA tag||MG50910-CY|
|Mouse PLAT Gene cDNA clone plasmid||MG50910-G|
|Mouse PLAT ORF mammalian expression plasmid, N-Flag tag||MG50910-NF|
|Mouse PLAT ORF mammalian expression plasmid, N-His tag||MG50910-NH|
|Mouse PLAT ORF mammalian expression plasmid, N-Myc tag||MG50910-NM|
|Mouse PLAT ORF mammalian expression plasmid, N-HA tag||MG50910-NY|
|Mouse PLAT natural ORF mammalian expression plasmid||MG50910-UT|
|Learn more about expression Vectors|
Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as α and β chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.