|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human LTC4S ORF mammalian expression plasmid, C-GFPSpark tag||HG11622-ACG|
|Human LTC4S ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11622-ACR|
|Human LTC4S ORF mammalian expression plasmid, N-GFPSpark tag||HG11622-ANG|
|Human LTC4S ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11622-ANR|
|Human LTC4S ORF mammalian expression plasmid, C-Flag tag||HG11622-CF|
|Human LTC4S ORF mammalian expression plasmid, C-His tag||HG11622-CH|
|Human LTC4S ORF mammalian expression plasmid, C-Myc tag||HG11622-CM|
|Human LTC4S ORF mammalian expression plasmid, C-HA tag||HG11622-CY|
|Human LTC4S Gene cDNA clone plasmid||HG11622-M|
|Human LTC4S ORF mammalian expression plasmid, N-Flag tag||HG11622-NF|
|Human LTC4S ORF mammalian expression plasmid, N-His tag||HG11622-NH|
|Human LTC4S ORF mammalian expression plasmid, N-Myc tag||HG11622-NM|
|Human LTC4S ORF mammalian expression plasmid, N-HA tag||HG11622-NY|
|Human LTC4S natural ORF mammalian expression plasmid||HG11622-UT|
|Learn more about expression Vectors|
Leukotriene C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S, is a multi-pass membrane protein which belongs to the MAPEG family. LTC4S is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelets. LTC4S is essential for the production of cysteinyl leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the conjugation function of human LTC4S has identified R51 and Y93 as critical for acid and base catalysis of LTA4 and reduced glutathione, respectively. A comparison across species for proteins that possess LTC4S activity reveals conservation of both of these residues, whereas R51 is absent in the FLAP molecules. Thus, within the glutathione S-transferase superfamily of genes, alignment of specific residues allows the separation of LTC4S family members from their most structurally similar counterparts, the FLAP molecules. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.