|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat APOA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80793-ACG|
|Rat APOA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80793-ACR|
|Rat APOA1 ORF mammalian expression plasmid, C-Flag tag||RG80793-CF|
|Rat APOA1 ORF mammalian expression plasmid, C-His tag||RG80793-CH|
|Rat APOA1 ORF mammalian expression plasmid, C-Myc tag||RG80793-CM|
|Rat APOA1 ORF mammalian expression plasmid, C-HA tag||RG80793-CY|
|Rat APOA1 ORF mammalian expression plasmid, N-Flag tag||RG80793-NF|
|Rat APOA1 ORF mammalian expression plasmid, N-His tag||RG80793-NH|
|Rat APOA1 ORF mammalian expression plasmid, N-Myc tag||RG80793-NM|
|Rat APOA1 ORF mammalian expression plasmid, N-HA tag||RG80793-NY|
|Rat APOA1 Gene cDNA clone plasmid||RG80793-U|
|Rat APOA1 natural ORF mammalian expression plasmid||RG80793-UT|
|Learn more about expression Vectors|
Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.