|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat AKR1A1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80786-ACG|
|Rat AKR1A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80786-ACR|
|Rat AKR1A1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80786-ANG|
|Rat AKR1A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80786-ANR|
|Rat AKR1A1 ORF mammalian expression plasmid, C-Flag tag||RG80786-CF|
|Rat AKR1A1 ORF mammalian expression plasmid, C-His tag||RG80786-CH|
|Rat AKR1A1 ORF mammalian expression plasmid, C-Myc tag||RG80786-CM|
|Rat AKR1A1 ORF mammalian expression plasmid, C-HA tag||RG80786-CY|
|Rat AKR1A1 ORF mammalian expression plasmid, N-Flag tag||RG80786-NF|
|Rat AKR1A1 ORF mammalian expression plasmid, N-His tag||RG80786-NH|
|Rat AKR1A1 ORF mammalian expression plasmid, N-Myc tag||RG80786-NM|
|Rat AKR1A1 ORF mammalian expression plasmid, N-HA tag||RG80786-NY|
|Rat AKR1A1 Gene cDNA clone plasmid||RG80786-U|
|Rat AKR1A1 natural ORF mammalian expression plasmid||RG80786-UT|
|Learn more about expression Vectors|
Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.