|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse L1CAM ORF mammalian expression plasmid, C-GFPSpark tag||MG50835-ACG|
|Mouse L1CAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50835-ACR|
|Mouse L1CAM ORF mammalian expression plasmid, C-Flag tag||MG50835-CF|
|Mouse L1CAM ORF mammalian expression plasmid, C-His tag||MG50835-CH|
|Mouse L1CAM ORF mammalian expression plasmid, C-Myc tag||MG50835-CM|
|Mouse L1CAM ORF mammalian expression plasmid, C-HA tag||MG50835-CY|
|Mouse L1CAM Gene cDNA clone plasmid||MG50835-G|
|Mouse L1CAM natural ORF mammalian expression plasmid, HA tag||MG50835-G-Y|
|Mouse L1CAM ORF mammalian expression plasmid, N-Flag tag||MG50835-NF|
|Mouse L1CAM ORF mammalian expression plasmid, N-His tag||MG50835-NH|
|Mouse L1CAM ORF mammalian expression plasmid, N-Myc tag||MG50835-NM|
|Mouse L1CAM ORF mammalian expression plasmid, N-HA tag||MG50835-NY|
|Mouse L1CAM natural ORF mammalian expression plasmid||MG50835-UT|
|Learn more about expression Vectors|
L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and in a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.