|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MAPK14 ORF mammalian expression plasmid, C-GFPSpark tag||MG50825-ACG|
|Mouse MAPK14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50825-ACR|
|Mouse MAPK14 ORF mammalian expression plasmid, N-GFPSpark tag||MG50825-ANG|
|Mouse MAPK14 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50825-ANR|
|Mouse MAPK14 ORF mammalian expression plasmid, C-Flag tag||MG50825-CF|
|Mouse MAPK14 ORF mammalian expression plasmid, C-His tag||MG50825-CH|
|Mouse MAPK14 ORF mammalian expression plasmid, C-Myc tag||MG50825-CM|
|Mouse MAPK14 ORF mammalian expression plasmid, C-HA tag||MG50825-CY|
|Mouse MAPK14 Gene cDNA clone plasmid||MG50825-G|
|Mouse MAPK14 ORF mammalian expression plasmid, N-Flag tag||MG50825-NF|
|Mouse MAPK14 ORF mammalian expression plasmid, N-His tag||MG50825-NH|
|Mouse MAPK14 ORF mammalian expression plasmid, N-Myc tag||MG50825-NM|
|Mouse MAPK14 ORF mammalian expression plasmid, N-HA tag||MG50825-NY|
|Mouse MAPK14 natural ORF mammalian expression plasmid||MG50825-UT|
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MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK14 can be detected in brain, heart, placenta, pancreas and skeletal muscle and it is expressed to a lesser extent in lung, liver and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. In respond to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide, MAPK14 phosphorylates a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example IL-6. It may play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.