|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50857-ACG|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50857-ACR|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-GFPSpark tag||MG50857-ANG|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50857-ANR|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-Flag tag||MG50857-CF|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-His tag||MG50857-CH|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-Myc tag||MG50857-CM|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, C-HA tag||MG50857-CY|
|Mouse MAPKAPK3 Gene cDNA clone plasmid||MG50857-G|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-Flag tag||MG50857-NF|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-His tag||MG50857-NH|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-Myc tag||MG50857-NM|
|Mouse MAPKAPK3 ORF mammalian expression plasmid, N-HA tag||MG50857-NY|
|Mouse MAPKAPK3 natural ORF mammalian expression plasmid||MG50857-UT|
|Learn more about expression Vectors|
The MAPKAP kinases are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is Widely expressed in human tissues, with a higher expression level observed in heart and skeletal muscle. No expression in brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines, and translocates to the cytoplasm from nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however the mechanism defining the specificity remains unknown.