|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TTR ORF mammalian expression plasmid, C-GFPSpark tag||RG80728-ACG|
|Rat TTR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80728-ACR|
|Rat TTR ORF mammalian expression plasmid, C-Flag tag||RG80728-CF|
|Rat TTR ORF mammalian expression plasmid, C-His tag||RG80728-CH|
|Rat TTR ORF mammalian expression plasmid, C-Myc tag||RG80728-CM|
|Rat TTR ORF mammalian expression plasmid, C-HA tag||RG80728-CY|
|Rat TTR ORF mammalian expression plasmid, N-Flag tag||RG80728-NF|
|Rat TTR ORF mammalian expression plasmid, N-His tag||RG80728-NH|
|Rat TTR ORF mammalian expression plasmid, N-Myc tag||RG80728-NM|
|Rat TTR ORF mammalian expression plasmid, N-HA tag||RG80728-NY|
|Rat TTR Gene cDNA clone plasmid||RG80728-U|
|Rat TTR natural ORF mammalian expression plasmid||RG80728-UT|
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Prealbumin/Transthyretin, also known as ATTR, Prealbumin, TTR and PALB, is a secreted and cytoplasm protein which belongs to the Prealbumin / Transthyretin family. Prealbumin / Transthyretin is detected in serum and cerebrospinal fluid (at protein level). It is highly expressed in choroid plexus epithelial cells. It is also detected in retina pigment epithelium and liver. Each monomer of Prealbumin / Transthyretin has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. Prealbumin/Transthyretin is a carrier protein. It transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. Defects in Prealbumin / Transthyretin are the cause of amyloidosis type 1 (AMYL1) which is a hereditary generalized amyloidosis due to Prealbumin / Transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.