|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD160 ORF mammalian expression plasmid, C-GFPSpark tag||MG50805-ACG|
|Mouse CD160 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50805-ACR|
|Mouse CD160 ORF mammalian expression plasmid, C-Flag tag||MG50805-CF|
|Mouse CD160 ORF mammalian expression plasmid, C-His tag||MG50805-CH|
|Mouse CD160 ORF mammalian expression plasmid, C-Myc tag||MG50805-CM|
|Mouse CD160 ORF mammalian expression plasmid, C-HA tag||MG50805-CY|
|Mouse CD160 Gene cDNA clone plasmid||MG50805-G|
|Mouse CD160 ORF mammalian expression plasmid, N-Flag tag||MG50805-NF|
|Mouse CD160 ORF mammalian expression plasmid, N-His tag||MG50805-NH|
|Mouse CD160 ORF mammalian expression plasmid, N-Myc tag||MG50805-NM|
|Mouse CD160 ORF mammalian expression plasmid, N-HA tag||MG50805-NY|
|Mouse CD160 natural ORF mammalian expression plasmid||MG50805-UT|
|Learn more about expression Vectors|
CD160 antigen, also known as Natural killer cell receptor BY55 and CD160, is a cell membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD160 is a GPI-anchored lymphocyte surface receptor in which expression is mostly restricted to the highly cytotoxic CD56(dim)CD16(+) peripheral blood NK subset. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed in spleen, peripheral blood, and small intestine. Expression of CD160 is restricted to functional NK and T cytotoxic lymphocytes. CD160 acts as a co-activator receptor for CD3-induced proliferation of CD4+ CD160+ T cells isolated from inflammatory skin lesions. Unique CD4+ CD160+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. Activated NK lymphocytes release a soluble form of CD160 that functionally impairs the MHC-I-specific cytotoxic CD8(+) T lymphocyte responsiveness.