|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse DDC ORF mammalian expression plasmid, C-GFPSpark tag||MG50799-ACG|
|Mouse DDC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50799-ACR|
|Mouse DDC ORF mammalian expression plasmid, N-GFPSpark tag||MG50799-ANG|
|Mouse DDC ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50799-ANR|
|Mouse DDC ORF mammalian expression plasmid, C-Flag tag||MG50799-CF|
|Mouse DDC ORF mammalian expression plasmid, C-His tag||MG50799-CH|
|Mouse DDC ORF mammalian expression plasmid, C-Myc tag||MG50799-CM|
|Mouse DDC ORF mammalian expression plasmid, C-HA tag||MG50799-CY|
|Mouse DDC Gene cDNA clone plasmid||MG50799-G|
|Mouse DDC ORF mammalian expression plasmid, N-Flag tag||MG50799-NF|
|Mouse DDC ORF mammalian expression plasmid, N-His tag||MG50799-NH|
|Mouse DDC ORF mammalian expression plasmid, N-Myc tag||MG50799-NM|
|Mouse DDC ORF mammalian expression plasmid, N-HA tag||MG50799-NY|
|Mouse DDC natural ORF mammalian expression plasmid||MG50799-UT|
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Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.